FIA of NADH Using Flow-Through Electrochemical Detector with Thionine-Adsorbed Carbon Felt
نویسندگان
چکیده
منابع مشابه
Carbon felt-based bioelectrocatalytic flow detectors: highly sensitive amperometric determination of hydrogen peroxide using adsorbed peroxidase and thionine.
Horseradish peroxidase (HRP) and thionine (TN) were co-adsorbed onto a porous carbon felt (CF), and the resulting HRP and TN-adsorbed CF (HRP-TN-CF) was successfully used as a working electrode unit of a novel bioelectrocatalytic flow detector for a highly sensitive amperometric determination of hydrogen peroxide (H(2)O(2)). Co-adsorbed TN was essential to enhance the cathodic peak current of H...
متن کاملCarbon-felt-based bioelectrocatalytic flow-detectors: optimization of the adsorption conditions of horseradish peroxidase and thionine onto carbon-felt for highly sensitive amperometric determination of H2O2.
Horseradish peroxidase (HRP) and thionine (Th) were co-adsorbed onto a porous carbon-felt (CF) from a mixed solution of HRP and Th to fabricate a CF-based bioelectrocatalytic flow-detector for H(2)O(2). The resulting HRP and Th co-adsorbed CF (HRP/Th-CF) was successfully used as a working electrode unit of a highly sensitive amperometric flow-H(2)O(2)-biosensor. Differing from the ordinary medi...
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Traditional vanadium batteries use pure sulfuric acid as electrolyte, but H2SO4 does not absorb enough vanadium ions to make the electrolyte an efficient energy source. This study investigates the effect of hydroxylation process on electrochemical and operational properties of carbon felt electrode in VOSO4 solution with an optimized supporting electrolyte (a mixture of six parts HCl and 2.5 pa...
متن کاملImmobilization of Tyrosinase on (3-Aminopropyl)triethoxysilane-Functionalized Carbon Felt-Based Flow-Through Detectors for Electrochemical Detection of Phenolic Compounds
Tyrosinase (TYR) was covalently immobilized onto amino-functionalized carbon felt (CF) surface via glutaraldehyde (GA). Prior to the TYR-immobilization, primary amino group was introduced to the CF surface by treatment with 3-aminopropyltriethoxysilane (APTES). The resulting TYR-immobilized CF was used as a working electrode unit of an electrochemical flow-through detector for monoand di-phenol...
متن کاملCarbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide
2,6-dichlorophenol indophenol (DCIP) and horseradish peroxidase (HRP) were coadsorbed on a porous carbon felt (CF) from their mixed aqueous solution under ultrasound irradiation for 5 min. The resulting DCIP and HRP-coadsorbed CF (DCIP/HRP-CF) showed an excellent bioelectrocatalytic activity for the reduction of H₂O₂. The coadsorption of DCIP together with HRP was essential to obtain larger bio...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 2005
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.54.1197